2 条结果 关键词:乳糖 ×

Nitrogen Doped Carbon Quantum Dots Modified by Lens culinaris β-Galactosidase as a Fluorescent Probe for Detection of Lactose.

Journal of fluorescence 2019 Singh R, Yadav A, Shekhar S, Ajad RK 等 6 人

本研究通过水热法以柠檬酸和尿素为碳源和氮源合成氮掺杂碳量子点(NCQDs)。TEM显示其近球形、平均粒径约1.5 nm,并具有良好的热稳定性和光学稳定性;FTIR、紫外-可见吸收和荧光光谱进一步证实其表面富含羧基且荧光发射峰位于约400 nm。随后采用EDC/NHS化学活化NCQDs表面羧基,并用BSA封闭非特异性位点,将扁豆β-半...

Safeguarding the catalytic activity and stability of polyaniline chitosan silver nanocomposite bound beta-galactosidase against product inhibitors and structurally related compound.

Artificial cells, nanomedicine, and biotechnology 2019 Khan M, Husain Q

本研究评估β-半乳糖苷酶(β-galactosidase, bGS)催化反应产物葡萄糖和半乳糖及其结构相关化合物维生素C(VC)对游离酶以及吸附于聚苯胺-壳聚糖纳米复合(PANI-CS-NC)和聚苯胺-壳聚糖-银纳米复合(PANI-CS-Ag-NC)上的固定化bGS催化活性的影响。结果表明,上述化合物均使可溶性酶活性下降,抑制顺序为...