An amperometric bienzymatic cholesterol biosensor based on functionalized graphene modified electrode and its electrocatalytic activity towards total cholesterol determination.
胆固醇氧化酶(ChOx)和胆固醇酯酶(ChEt)通过共价方式固定在羧基功能化石墨烯(FG)修饰的石墨电极上,用于游离胆固醇和总胆固醇的电化学检测。循环伏安法和电化学阻抗谱表明,FG可促进电极与固定ChOx之间的电子转移,实现ChOx中FAD/FADH2的直接电子转移,电子转移系数α为0.31,电子转移速率常数Ks为0.78 s−1。...