1 条结果 关键词:动物传染病 ×

CRISPR/Cas system and its application in the diagnosis of animal infectious diseases.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Rasool HMH, Chen Q, Gong X, Zhou J

传染病严重威胁人与动物健康,现有诊断方法常依赖昂贵设备、专业实验室,且存在耗时、灵敏度和特异性不足等问题,难以满足大规模现场检测需求。近年来,成簇规律间隔短回文重复(CRISPR)诊断平台凭借超灵敏核酸检测能力,推动了分子诊断领域变革。本文综述了不同 CRISPR/Cas 系统的类型、Cas 蛋白特性及核酸扩增策略,重点介绍 CRI...