Colorimetric detection of genetically modified organisms based on exonuclease III-assisted target recycling and hemin/G-quadruplex DNAzyme amplification.

描述了一种等温比色方法，用于转基因生物（GMO）中CaMV 35S启动子序列的放大检测。该方法基于：（a）靶DNA触发的无标记分子信标（UMB）末端结合；（b）外切酶III（Exo III）辅助靶标循环；（c）血红素/G-四链体（DNAzyme）信号放大。靶标与G-四链体序列锁定的UMB特异性结合后触发Exo III消化，释放活性G...