Evaluation of molecularity of rate-limiting step of pore formation by antimicrobial peptides studied using mitochondria as a biosensor.

来自蜂毒、黄蜂毒和真菌的毒性物质蜂毒肽（melittin）、黄蜂毒肽（mastoparan）和短杆菌肽（alamethicin）可通透生物膜。本研究以能产生跨膜电位（ΔΨ）的大鼠肝线粒体（RLM）为钾跨膜电流（PTC）传感器，考察这些肽在RLM中形成孔的初始步骤。在含6 mM Mg2+的“钾”孵育介质中，melittin诱导的RLM...