Accelerated strain construction and characterization of C. glutamicum protein secretion by laboratory automation.
细菌蛋白分泌到培养上清可简化下游纯化,但高效分泌需要筛选信号肽与目标蛋白组合,且受表达效率和培养条件影响。本研究建立两套自动化工作流,用于谷氨酸棒杆菌(Corynebacterium glutamicum)中腐皮镰刀菌梨孢型(Fusarium solani f. sp. pisi)角质酶的异源分泌菌株构建与高通量筛选。利用 Open...