1 条结果 关键词:无酶免疫检测 ×

Development of an optical immunoassay based on peroxidase-mimicking Prussian blue nanoparticles and a label-free electrochemical immunosensor for accurate and sensitive quantification of milk species adulteration.

Mikrochimica acta 2022 Seddaoui N, Attaallah R, Amine A

本文首次报道一种无酶免疫检测平台,用于以牛IgG作为山羊奶掺假标志物的现场检测。第一种为光学比色竞争微孔板免疫检测,普鲁士蓝纳米颗粒(PBNPs)作为抗体催化标记,替代天然过氧化物酶,在450 nm处进行吸光度测定。经优化后,该方法对牛/羊奶体积比达到0.01%的灵敏度,比欧盟法规允许的1%低100倍;抗体-PBNPs偶联物在4周内...