Enzymatic amplification-free nucleic acid hybridisation sensing on nanostructured thick-film electrodes by using covalently attached methylene blue.
无酶放大检测因流程快速、简单而受到生物传感器开发重视,但保持灵敏度需要高可检测标记物或高灵敏换能技术。本文以氨基化多壁碳纳米管(MWCNT-NH2)、金纳米颗粒(AuNPs)或二者纳米杂化结构修饰金丝网印刷电极(AuSPEs),用于DNA杂交传感。亚甲基蓝(MB)共价连接于单链DNA(ssDNA)末端,与电极表面固定的互补序列孵育,...