A label-free fluorescent direct detection of live Salmonella typhimurium using cascade triple trigger sequences-regenerated strand displacement amplification and hairpin template-generated-scaffolded silver nanoclusters.
鼠伤寒沙门氏菌(Salmonella typhimurium, S. typhimurium)通过污染农产品或水传播,威胁公共卫生,亟需快速检测方法。本文首次构建一种无标记荧光生物传感器,利用三重触发序列再生链置换扩增(T-SDA)和自保护发夹模板生成支架化银纳米团簇(AgNCs)。活菌存在时,鼠伤寒沙门氏菌适配体与菌体结合,使适配...