Electrochemical Genosensor To Detect Pathogenic Bacteria (Escherichia coli O157:H7) As Applied in Real Food Samples (Fresh Beef) To Improve Food Safety and Quality Control.

电化学生物传感器是快速、可靠检测致病菌的有前景方法。作者此前曾利用硫化铅纳米颗粒（PbSNPs）构建电化学基因传感器检测金黄色葡萄球菌。本研究将该策略用于检测致病性大肠杆菌O157:H7。引物和探针基于NCBI数据库及Sigma-Aldrich软件设计，捕获探针和信号探针分别以巯基（SH）和氨基（NH2）修饰；信号探针进一步偶联硫化...