"Molecular beacon"-directed fluorescence of Hoechst dyes for visual detection of Hg(II) and biothiols and its application for a logic gate.
本研究开发了一种分子信标样DNA发夹生物传感器,利用Hoechst染料对富AT双链DNA小沟结合后的荧光增强效应,实现Hg2+和生物硫醇的无标记荧光检测。ATprobe在Hg2+诱导下形成T-Hg2+-T茎环发夹,为Hoechst染料提供结合位点,使其荧光由弱变强;随后Cys、Hcys、GSH等生物硫醇通过硫醇-Hg2+作用夺取Hg...