A rapid method to detect and estimate the activity of the enzyme, alcohol oxidase by the use of two chemical complexes - acetylacetone (3,5-diacetyl-1,4-dihydrolutidine) and acetylacetanilide (3,5-di-N-phenylacetyl-1,4-dihydrolutidine).

本研究开发了一种快速、灵敏的方法，用于检测并估算真菌合成醇氧化酶（alcohol oxidase, AOX）的活性。该方法利用AOX特异性氧化甲醇，生成等摩尔的甲醛（HCHO）和过氧化氢（H2O2）。一方面，甲醛与中性乙酰丙酮及铵盐溶液反应，逐渐生成黄色3,5-二乙酰-1,4-二氢吡啶（λ=420 nm；λex/em=390/470...