Highly sensitive fluorescence biosensor for intracellular telomerase detection based on a single patchy gold/carbon nanosphere via the combination of nanoflare and hybridization chain reaction.

本文针对细胞内端粒酶活性原位高灵敏检测的难题，构建了一种基于单个斑块状金/碳纳米球（PG/CNS）的荧光生物传感平台。该平台将纳米flare与杂交链反应（HCR）信号放大策略集成于同一Janus结构纳米颗粒上：碳纳米球表面的金斑块通过Au–S键选择性结合杂交DNA（HS-DNA/Primer-DNA/Flare-DNA），而一对交叉...