2 条结果 关键词:肺炎克雷伯菌 ×

Isothermal amplification and rapid detection of Klebsiella pneumoniae based on the multiple cross displacement amplification (MCDA) and gold nanoparticle lateral flow biosensor (LFB).

PloS one 2018 Niu L, Zhao F, Chen J, Nong J 等 10 人

肺炎克雷伯菌(Klebsiella pneumoniae, K. pneumoniae)是引起院内感染和暴发的重要病原菌。本研究建立了一种基于多重交叉置换扩增(multiple cross displacement amplification, MCDA)的K. pneumoniae快速检测方法,可在65°C等温扩增40 min内获...

Rapid, sensitive and reliable detection of Klebsiella pneumoniae by label-free multiple cross displacement amplification coupled with nanoparticles-based biosensor.

Journal of microbiological methods 2018 Wang Y, Yan W, Wang Y, Xu J 等 5 人

肺炎克雷伯菌(Klebsiella pneumoniae)是重要的医院获得性细菌,可导致老年人、新生儿和免疫受损人群严重发病和死亡。本研究建立了一种基于rcsA基因的无标记多重交叉置换扩增(MCDA)结合侧流生物传感器(LFB)方法,用于快速、简便、灵敏地检测肺炎克雷伯菌。MCDA反应在65°C固定温度下进行30 min,扩增结果直...