Measurement of the nuclear concentration of α-ketoglutarate during adipocyte differentiation by using a fluorescence resonance energy transfer-based biosensor with nuclear localization signals.

α-酮戊二酸（α-KG，又称 2-氧代戊二酸/2-OG）是三羧酸循环中间代谢物，也是谷氨酸脱氨产物，并为包括 TET DNA 去甲基化酶和 JMJD 组蛋白去甲基化酶在内的 2-OG 依赖性加氧酶提供必需辅因子。由于这些表观遗传酶作用于细胞核内基因组 DNA 和组蛋白，核内 α-KG 浓度可能通过调节酶活性影响转录水平。本文报道一种...