Label-free colorimetric biosensing of copper(II) ions with unimolecular self-cleaving deoxyribozymes and unmodified gold nanoparticle probes.
本文基于单分子铜(II)依赖自切割脱氧核酶(DNAzyme)和未修饰金纳米颗粒(AuNPs)构建了一种无标记比色生物传感器,用于检测铜(II)离子(Cu2+)。该传感器利用单链DNA(ssDNA)在AuNP表面吸附的序列长度依赖性:在Cu2+存在时,Cu2+依赖DNAzyme发生自切割,生成短ssDNA片段;短片段可快速吸附到AuN...