Design of a Whole-Cell Biosensor Based on Bacillus subtilis Spores and the Green Fluorescent Protein To Monitor Arsenic.
本研究在枯草芽孢杆菌(Bacillus subtilis)中构建了一种基于绿色荧光蛋白(GFP)的全细胞生物传感器(WCB-GFP),用于监测砷(As)。作者将 gfpmut3a 基因置于砷操纵子启动子/操纵区(Pars)控制下,构建到质粒 pAD123 中,并转化至 B. subtilis 168,获得菌株 BsWCB-GFP。该...