4 条结果 关键词:适配体 ×

Brevetoxin Aptamer Selection and Biolayer Interferometry Biosensor Application.

Toxins 2024 Hu B, Ouyang SQ, Zhu YP, Lu XL 等 9 人

贝类毒素(PbTxs)是强效海洋神经毒素,可致神经性贝类中毒(NSP),其环多醚结构在过去20年地理分布扩大。现有检测方法存在伦理问题和技术局限,亟需替代方法用于监管监测。本研究首次同时筛选针对PbTx-1和PbTx-2的DNA适配体,并以PbTx-1适配体为识别元件构建生物层干涉(BLI)生物传感器。通过体外筛选,从随机序列库中成...

Author Correction: Cell-SELEX for aptamer discovery and its utilization in constructing electrochemical biosensor for rapid and highly sensitive detection of Legionella pneumophila serogroup 1.

Scientific reports 2024 Shaukat A, Chrouda A, Sadaf S, Alhamlan F 等 6 人

本文为 Scientific Reports 原论文的作者更正。原论文题为“Cell-SELEX for aptamer discovery and its utilization in constructing electrochemical biosensor for rapid and highly sensitive det...

Rapid and Sensitive Detection of Inactivated SARS-CoV-2 Virus via Fiber-Optic and Electrochemical Impedance Spectroscopy Based Aptasensors.

Biosensors 2024 Xiao C, Wang N, Zhao Y, Liu X 等 10 人

快速病毒检测工具对防控大流行和生物威胁至关重要。针对灭活病毒的适配体因生物安全性高而适合用于传感器。本文首次评估了一种特异性结合灭活SARS-CoV-2的DNA适配体6.9,其解离常数KD为9.6 nM。基于适配体6.9,作者构建了光纤倏逝波(FOEW)生物传感器:灭活SARS-CoV-2与Cy5.5标记的短互补链竞争结合固定在传感...

Ultrasensitive detection of vital biomolecules based on a multi-purpose BioMEMS for Point of care testing: digoxin measurement as a case study.

Scientific reports 2024 Marvi F, Jafari K, Shahabadi M, Tabarzad M 等 6 人

为实现慢病诊断和治疗药物监测中低浓度生物标志物的快速无标记检测,本文提出一种多用途光学生物微机电系统(BioMEMS)传感平台。该平台以高灵敏度MEMS悬臂梁为换能器,利用其表面应力响应将生物识别事件转换为机械位移,并通过可调光学干涉系统、CCD相机和图像处理算法精确记录纳米级位移,从而识别分析物类型并定量其浓度。通过更换生物受体并...