An ultrasensitive and specific point-of-care CRISPR/Cas12 based lateral flow biosensor for the rapid detection of nucleic acids.
CRISPR/Cas系统因Cas效应蛋白(如Cas12、Cas13)的伴随切割活性,在核酸检测生物传感器中展现出巨大潜力。然而,现有CRISPR/Cas检测平台通常依赖核酸扩增、昂贵荧光仪器或侧流层析试纸条,限制了现场应用。本文报道了一种简单、低成本、超灵敏的DNA探针侧流层析生物传感器(LFB),将CRISPR/Cas与环介导等温...