Visualization of Ins(1,4,5)P3 dynamics in living cells: two distinct pathways for Ins(1,4,5)P3 generation following mechanical stimulation of HSY-EA1 cells.

本研究在HSY-EA1细胞中考察肌醇(1,4,5)-三磷酸[Ins(1,4,5)P3]生成对机械刺激诱导Ca2+响应的贡献。利用Ins(1,4,5)P3生物传感器LIBRAvIII，机械刺激引起受刺激区域胞浆[IP3]i局部升高，并随刺激区[IP3]i升高而像胞内IP3波一样扩展；随后邻近细胞出现短暂小幅[IP3]i升高。磷脂酶C抑...