Ultrasensitive Homogeneous Electrochemiluminescence Biosensor for a Transcription Factor Based on Target-Modulated Proximity Hybridization and Exonuclease III-Powered Recycling Amplification.
本文报道了一种用于检测转录因子 NF-κB p50 的超灵敏均相电化学发光(ECL)生物传感器。该传感器基于靶标调控的邻近杂交与外切酶 III(Exo III)驱动的循环放大策略。Ru(bpy)3^2+ 标记的发夹 DNA(HP-Rul)因 DNA 骨架带大量负电荷,难以接近带负电的 ITO 电极,因而背景 ECL 信号弱。DNA1...