Real time analysis of the RNAI-RNAII-Rop complex by surface plasmon resonance: from a decaying surface to a standard kinetic analysis.
RNA环-环复合物是原核和真核生物中调控生物学功能的重要结构基序。在大肠杆菌中,ColE1质粒编码的反义RNA RNAI通过环-环相互作用识别RNAII,后者是结合质粒DNA并启动复制的RNA引物,从而调控质粒复制。质粒编码的同源二聚体蛋白Rop可与这一瞬时RNAI-RNAII kissing complex相互作用。本研究采用表面...