Preparation of extracellular domain 3 of human VEGF receptor-2 and the monitoring of its real-time binding to VEGF by biosensors.

本研究报道了人血管内皮生长因子受体-2（VEGFR-2）胞外域3（KDR3）的高表达、纯化及其与VEGF实时结合的生物传感器检测。重组KDR3在大肠杆菌中表达，经阳离子交换色谱和固定化金属亲和色谱（IMAC）纯化，纯度约95%，产量26 mg/L。通过人脐静脉内皮细胞（HUVEC）增殖实验验证其生物学活性，700 ng/mL KDR...