Altered agonist sensitivity of a mutant v2 receptor suggests a novel therapeutic strategy for nephrogenic diabetes insipidus.

肾性尿崩症（NDI）可由肾脏2型血管加压素受体（V2R）功能缺失突变引起。本研究鉴定一名NDI患者中已报道但未表征的V2R N321K错义突变。作者构建高灵敏度Epac型生物发光共振能量转移（BRET）生物传感器，在激动剂刺激瞬时转染V2R的HEK293细胞后实时测量cAMP；并用BRET检测β-arrestin结合，用抗HA-Al...