Robust and highly specific fluorescence sensing of Salmonella typhimurium based on dual-functional phi29 DNA polymerase-mediated isothermal circular strand displacement polymerization.

本文报道了一种用于病原菌检测的简单、稳健的荧光传感策略，该策略将phi29 DNA聚合酶的双重功能与等温环状链置换聚合（ICSDP）相结合。检测依赖目标物触发成熟引物的形成，在双功能phi29辅助下启动环状链置换聚合反应，从而实现目标物的放大检测。据作者所知，这是首次将双功能phi29辅助ICSDP用于病原菌的荧光传感。策略中引入发...