Analysis of antibody-antigen interactions using surface plasmon resonance.
Accurate analyses of antibody specificities and affinities are critical to understanding their role in antibody-mediated neutralization of HIV-1 in vitro and their pot...
On-line heat flux measurements improve the culture medium for the growth and productivity of genetically engineered CHO cells.
With the increasingly competitive commercial production of target proteins by hybridoma and genetically engineered cells, there is an urgent requirement for biosensors...
A Novel Catechol Oxidase Enzyme Electrode for the Specific Determination of Catechol.
An enzyme electrode for the specific determination of catechol was developed by using catechol oxidase (EC 1.10.3.1) from eggplant (Solanum melangena L.) in combinatio...
Thionine covalently tethered to multilayer horseradish peroxidase in a self-assembled monolayer as an electron-transfer mediator.
A new approach to construct a reagentless enzyme biosensor is described. Based on multilayer horseradish peroxidase in a self-assembled monolayer configuration, the bi...
A Fill-and-Flow Biosensor.
An alternative design for an amperometric biosensor for lactate is described in which the analyte flows through a rectangular duct without the aid of external pumping ...
Biosensor-based methods in clinical diagnosis.
Despite the multitude of different parameters currently measured in the clinical laboratory, only a minor part of these are measured by means of biosensor-based method...
Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones.
Quorum sensing relies upon the interaction of a diffusible signal molecule with a transcriptional activator protein to couple gene expression with cell population dens...
A microscale biosensor for methane containing methanotrophic bacteria and an internal oxygen reservoir.
A microscale biosensor for continuous measurement of methane partial pressure based on a novel counterdiffusion principle is presented. Methane-oxidizing bacteria plac...
Microfabricated electrochemical sensor for the detection of radiation-induced DNA damage.
An electrochemical biosensor protocol for the detection of radiation-induced DNA damage is described. The procedure employs a dsDNA-coated screen-printed electrode and...
An amperometric fructose biosensor based on fructose dehydrogenase immobilized in a membrane mimetic layer on gold.
A prototype amperometric fructose biosensor based on membrane-bound fructose dehydrogenase (Gluconobacter sp.) and the coenzyme ubiquinone-6 immobilized in a membrane ...
A Microscale NO(3)(-) Biosensor for Environmental Applications.
A biosensor for NO(3)(-) containing immobilized dentrifying bacteria and a reservoir of liquid growth medium for the bacteria was constructed. The bacteria did not hav...
Graphite-Poly(tetrafluoroethylene) Composite Enzyme Electrodes as Suitable Biosensors in Predominantly Nonaqueous Media.
The performance of a graphite-poly(tetrafluoroethylene) Teflon composite amperometric ferrocyanide-mediated peroxidase electrode in a predominantly nonaqueous medium s...
LC-Biosensor System for the Determination of the Neurotoxin β-N-Oxalyl-l-α,β-diaminopropionic Acid.
An analysis system is described for the determination of the neurotoxin β-N-oxalyl-l-α,β-diaminopropionic acid (β-ODAP). The system is based on liquid chromatographic ...
Composite transducers for amperometric biosensors. The glucose sensor.
A new concept of a composite transducer for amperometric biosensors based on the use of a solid substance with amphiphilic character (called a solid binding matrix, SB...
Reagentless mediated laccase electrode for the detection of enzyme modulators.
We have investigated aerobic mediation of electron transfer to a laccase enzyme by the solution redox couples [Os(bpy)(2)Cl(2)](+/0) and [Os(bpy)(2)(MeIm)Cl](2+/+), wh...
Mechanistic Studies of the Electrocatalytic Oxidation of NADH and Ascorbate at Glassy Carbon Electrodes Modified with Electrodeposited Films Derived from 3,4-Dihydroxybenzaldehyde.
Studies of the electrocatalytic oxidation of β-nicotinamide adenine dinucleotide (NADH) at glassy carbon rotated disk electrodes modified with electrodeposited films d...
An optical biosensor study of the interaction parameters and role of hydrophobic tails of cytochrome P450 2B4, b5 and NADPH-flavoprotein in complex formation.
The real-time interactions of membrane proteins - cytochrome P450 2B4, NADPH cytochrome P450 reductase and cytochrome b5 - were studied by use of an optical biosensor ...
Comparison of Biosensor, Microbiological, Immunochemical, and Physical Methods for Detection of Sulfamethazine Residues in Raw Milk.
A biosensor assay based on biospecific interaction analysis (BIA) was compared with already existing methods for detection of sulphamethazine (SMZ) residues in milk. M...
DNA biosensor for the detection of hydrazines.
A double-stranded (ds) DNA-coated carbon paste electrode is employed as a remarkably sensitive biosensor for the detection of hydrazine compounds. The sensor relies on...