4 条结果 关键词:β-半乳糖苷酶 ×

Nitrogen Doped Carbon Quantum Dots Modified by Lens culinaris β-Galactosidase as a Fluorescent Probe for Detection of Lactose.

Journal of fluorescence 2019 Singh R, Yadav A, Shekhar S, Ajad RK 等 6 人

本研究通过水热法以柠檬酸和尿素为碳源和氮源合成氮掺杂碳量子点(NCQDs)。TEM显示其近球形、平均粒径约1.5 nm,并具有良好的热稳定性和光学稳定性;FTIR、紫外-可见吸收和荧光光谱进一步证实其表面富含羧基且荧光发射峰位于约400 nm。随后采用EDC/NHS化学活化NCQDs表面羧基,并用BSA封闭非特异性位点,将扁豆β-半...

Conjugation of Carbon Dots with β-Galactosidase Enzyme: Surface Chemistry and Use in Biosensing.

Molecules (Basel, Switzerland) 2019 Sharma SK, Micic M, Li S, Hoar B 等 7 人

纳米颗粒已与多种生物系统偶联,用于自组装、传感、成像和治疗。开发响应快、检测限低、使用寿命长的可靠稳健生物传感器需求迫切。本文通过偶联关键酶β-半乳糖苷酶与实验室合成的凝胶状碳点(CDs)构建传感平台。该碳点具有高发光、光稳定性和易表面功能化特点。研究发现,共轭酶对温度和pH变化的稳定性显著高于天然酶。利用这一增强性质,作者开发了稳...

Carbon nanotubes molybdenum disulfide 3D nanocomposite as novel nanoscaffolds to immobilize Lens culinaris β-galactosidase (Lsbgal): Robust stability, reusability, and effective bioconversion of lactose in whey.

Food chemistry 2019 Yadav A, Pandey SK, Agrawal DC, Mishra H 等 6 人

本文通过环保水热法合成多壁碳纳米管-二硫化钼三维纳米复合材料(MWCNT-MoS2 NC),并采用光谱与显微技术表征其微观结构。该纳米复合材料经戊二醛活化后,作为固定化 Lens culinaris β-半乳糖苷酶(Lsbgal)的平台,固定化效率达 93%。原子力显微镜、场发射扫描电镜、傅里叶变换红外光谱和共聚焦激光扫描显微镜证实...

Safeguarding the catalytic activity and stability of polyaniline chitosan silver nanocomposite bound beta-galactosidase against product inhibitors and structurally related compound.

Artificial cells, nanomedicine, and biotechnology 2019 Khan M, Husain Q

本研究评估β-半乳糖苷酶(β-galactosidase, bGS)催化反应产物葡萄糖和半乳糖及其结构相关化合物维生素C(VC)对游离酶以及吸附于聚苯胺-壳聚糖纳米复合(PANI-CS-NC)和聚苯胺-壳聚糖-银纳米复合(PANI-CS-Ag-NC)上的固定化bGS催化活性的影响。结果表明,上述化合物均使可溶性酶活性下降,抑制顺序为...