Direct detection and discrimination of double-stranded oligonucleotide corresponding to hepatitis C virus genotype 3a using an electrochemical DNA biosensor based on peptide nucleic acid and double-stranded DNA hybridization.

本文报道了一种基于肽核酸（PNA）探针和双链DNA杂交的电化学生物传感器，用于直接检测并区分对应丙型肝炎病毒（HCV）3a基因型core/E1区的双链寡核苷酸（dsDNA），无需对目标DNA进行变性。传感器以多晶金盘电极为基底，将14-mer PNA探针pHCV3a自组装于金表面，并用6-巯基-1-己醇（MCH）封闭。当目标dsDN...