2 条结果 关键词:丙型肝炎病毒3a ×

Direct detection and discrimination of double-stranded oligonucleotide corresponding to hepatitis C virus genotype 3a using an electrochemical DNA biosensor based on peptide nucleic acid and double-stranded DNA hybridization.

Analytical and bioanalytical chemistry 2010 Pournaghi-Azar MH, Ahour F, Hejazi MS

本文报道了一种基于肽核酸(PNA)探针和双链DNA杂交的电化学生物传感器,用于直接检测并区分对应丙型肝炎病毒(HCV)3a基因型core/E1区的双链寡核苷酸(dsDNA),无需对目标DNA进行变性。传感器以多晶金盘电极为基底,将14-mer PNA探针pHCV3a自组装于金表面,并用6-巯基-1-己醇(MCH)封闭。当目标dsDN...

Electrochemical detection of short sequences of hepatitis C 3a virus using a peptide nucleic acid-assembled gold electrode.

Analytical biochemistry 2010 Hejazi MS, Pournaghi-Azar MH, Ahour F

本文报道了一种基于肽核酸(PNA)自组装金电极的电化学DNA生物传感器,用于检测丙型肝炎病毒基因型3a(HCV-3a)core/E1区短序列。传感器以多晶金电极为基底,将含半胱氨酸锚定基团的14-mer PNA探针(pHCV3a)通过硫醇-金共价作用自组装固定,并用6-巯基-1-己醇(MCH)封闭以去除非特异吸附和填充针孔。该PNA...