Establishment and Application of a Multiple Cross Displacement Amplification Coupled With Nanoparticle-Based Lateral Flow Biosensor Assay for Detection of

Frontiers in cellular and infection microbiology 2019 Wang Y, Wang Y, Quan S, Jiao W 等 10 人

肺炎支原体（Mycoplasma pneumoniae, M. pneumoniae）是肺炎及支气管炎等呼吸道感染的重要病原体。本研究建立并应用多重交叉置换扩增（multiple cross displacement amplification, MCDA）耦合纳米颗粒侧流生物传感器（lateral flow biosensor, ...

侧流层析试纸条 肺炎支原体多重交叉置换扩增侧流层析生物传感器纳米颗粒

Detection of Nucleic Acids and Prevention of Carryover Contamination Using Cross-Priming Amplification Combined with Nanoparticles-Based Biosensor and Antarctic Thermal Sensitive Uracil-DNA-Glycosylase.

Journal of biomedical nanotechnology 2019 Wang Y, Sun L, Li JQ, Wang ZM 等 12 人

本研究报道了一种交叉引物扩增（CPA）方案，利用南极热敏感尿嘧啶-DNA糖基化酶（AUDG）同时实现核酸检测与防污染。扩增产物通过纳米颗粒侧流生物传感器（LFB）检测。该方法仅需一条标记引物，无需标记探针，可消除两条标记引物之间或探针与标记引物之间非特异杂交导致的假阳性。CPA扩增与AUDG切割在同一管中进行，密闭反应体系可避免气溶...

侧流层析试纸条 交叉引物扩增侧流层析生物传感器尿嘧啶-DNA糖基化酶肺炎克雷伯菌