2 条结果 关键词:自避免分子识别系统 ×

A label-free technique for accurate detection of nucleic acid-based self-avoiding molecular recognition systems supplemented multiple cross-displacement amplification and nanoparticles based biosensor.

Artificial cells, nanomedicine, and biotechnology 2018 Wang Y, Wang Y, Wang H, Xu J 等 5 人

本研究基于标准多重交叉置换扩增(MCDA)开发了一种新型等温核酸检测技术,并引入自避免分子识别系统(SAMRS)组分与南极热敏感尿嘧啶-DNA糖基化酶(AUDG),命名为AUDG–SAMRS–MCDA。为实现扩增产物在试纸条上的检测,作者建立了无需标记引物或探针的分析策略,从而避免标记引物之间或标记探针与引物之间非预期杂交造成的假阳...

Loop-mediated isothermal amplification using self-avoiding molecular recognition systems and antarctic thermal sensitive uracil-DNA-glycosylase for detection of nucleic acid with prevention of carryover contamination.

Analytica chimica acta 2017 Wang Y, Liu D, Deng J, Wang Y 等 6 人

环介导等温扩增(LAMP)无需温度循环即可扩增核酸,但常因引物间二聚体、非靶杂交和携带污染导致假阳性。本文建立一种由自避免分子识别系统(SAMRS)组分和南极热敏感尿嘧啶-DNA糖基化酶(AUDG)辅助的新型LAMP方法,称为AUDG-SAMRS-LAMP。将SAMRS组分引入LAMP引物3′端可提高特异性,完全避免由非靶杂交及引物...