A label-free technique for accurate detection of nucleic acid-based self-avoiding molecular recognition systems supplemented multiple cross-displacement amplification and nanoparticles based biosensor.
本研究基于标准多重交叉置换扩增(MCDA)开发了一种新型等温核酸检测技术,并引入自避免分子识别系统(SAMRS)组分与南极热敏感尿嘧啶-DNA糖基化酶(AUDG),命名为AUDG–SAMRS–MCDA。为实现扩增产物在试纸条上的检测,作者建立了无需标记引物或探针的分析策略,从而避免标记引物之间或标记探针与引物之间非预期杂交造成的假阳...