3 条结果 关键词:酶纳米颗粒 ×

An improved amperometric creatinine biosensor based on nanoparticles of creatininase, creatinase and sarcosine oxidase.

Analytical biochemistry 2017 Kumar P, Jaiwal R, Pundir CS

本文报道了一种基于肌酸酐酶(CA)、肌酸酶(CI)和肌氨酸氧化酶(SOx)纳米颗粒(ENPs)的改进型安培法肌酸酐生物传感器。三种酶分别经乙醇去溶剂化、戊二醛交联和半胱胺二盐酸盐氨基化制备成纳米颗粒,并以1:1:1比例固定于经清洗的玻璃碳(GC)电极表面。采用TEM、FTIR、SEM、CV和EIS对酶纳米颗粒及电极进行表征。在0.1...

An improved amperometric triglyceride biosensor based on co-immobilization of nanoparticles of lipase, glycerol kinase and glycerol 3-phosphate oxidase onto pencil graphite electrode.

Enzyme and microbial technology 2017 Narwal V, Pundir CS

本研究制备并表征了来自Candida rugosa的脂肪酶、来自Cellulomonas属的甘油激酶(GK)和来自Aerococcus viridans的甘油-3-磷酸氧化酶(GPO)的酶纳米颗粒(ENPs),并将其共固定于铅笔石墨(PG)电极表面。通过扫描电镜(SEM)、循环伏安(CV)和电化学阻抗谱(EIS)对固定前后电极进行形...

Amperometric triglyceride bionanosensor based on nanoparticles of lipase, glycerol kinase, glycerol-3-phosphate oxidase.

Analytical biochemistry 2017 Pundir CS, Aggarwal V

本研究以猪胰腺脂酶、Cellulomonas sp. 甘油激酶(GK)和 Aerococcus viridans 甘油-3-磷酸氧化酶(GPO)为原料,通过乙醇除溶剂和戊二醛交联制备酶纳米颗粒(ENPs),并用半胱胺功能化引入巯基。TEM 和 FTIR 表征了 ENPs 的形貌与结构。功能化 ENPs 通过 Au-S 键共价共固定于...