Capture antibody targeted fluorescence in situ hybridization (CAT-FISH): dual labeling allows for increased specificity in complex samples.

病原菌生物传感器检测常受混合群体中敏感性和特异性限制。本文开发一种双标记方法，使液相荧光原位杂交（FISH）与捕获抗体靶向检测（CAT-FISH）相结合。以大肠杆菌O157:H7和金黄色葡萄球菌为代表菌，评估处理工艺对FISH荧光强度和抗体识别的影响。替代固定液methacarn优于标准固相多聚甲醛（PFA）固定，可同时保留FISH...