Generation of an anti-NAGase single chain antibody and its application in a biosensor-based assay for the detection of NAGase in milk.

牛乳腺炎是奶牛业重大挑战，早期诊断对降低产量损失、提高奶质和控制成本至关重要。N-乙酰-β-D-氨基己糖苷酶（NAGase）在乳腺炎症时释放入奶，可作为乳腺炎早期指标。本文从人源Tomlinson单链可变片段（scFv）抗体文库中筛选抗NAGase scFv，并将其用于自动化光学生物传感器免疫分析以检测牛奶中NAGase。经噬菌体展...